"

From 2011.igem.org

28th of July

-We have made tryptone broth, which will be used to grow E. coli (K-12 D10b strain) overnight before the chemotaxis
experiments, for them to develop flagella and therefore they will be capable of chemotaxis. To make tryptone broth look
at protocols.

-Cells have been transformed with backbone plasmid pSB1C3 carrying biobrick BBa_K398500, with constitutive promoter J23100,
protocol for transformation can be found here.