Team:British Columbia/Notebook/Week 8

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On July 25th, Gurpal performed a yeast transformation. He transformed his purified pBS and pKS into 2 separate wildtype yeasts. He followed a protocol, left the cells to incubate at 30 degrees Celsius at 3:10pm, heat shocked them for 20 minutes at 42 degrees Celsius and then plated them at 5:30pm. The plates were marked with URA so that the plasmids could grow on them. Then, they were incubated in a 30 degree Celsius incubator.

On July 27th, Alina observed the cells and noted that the transformation was a success.