"
Team:Lyon-INSA-ENS/Realisation/Week5
From 2011.igem.org
Week 5
From Monday the 4th of July to Friday the 8th of July 2011
Monday
Additional minipreps of the same 6 parts. Digestion and ligation to obtain : RBS ( strong and weak ) + GFP, RBS + YFP. Culture of NM522 competent cells.
Tuesday
Transformation of the ligations into NM522 using a CaCl2 chemical transformation.
Wednesday
Selection of individual transformed colonies and start of solid and liquid culture.
We shoot our "Cobalt Buster's Clip" which introduces, in a funny way, our team : students, instructors and advisors. We dedicate the all afternoon to this shooting.
Thursday
Extraction, digestion and electrophoresis of the selected transformed cultures of individual colonies : the transformation had failed.
