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July 24
Started overnight cultures of pSB3K3
July 25
PCR'd parts for pNT002: R0040, K123001, B0014, pSB4A5
PCR'd GFP control insert and pNT003 insert
Gibson assembly and transformation of pNT003 with a negative control
Results
Decided not to use the pNT003 PCR'd insert, as the band of the correct length was much fainter than a lower length band. Chose not to do Gibson assembly of the positive GFP control, despite not having any green colonies last week, instead focusing on assembling pNT002 and pNT003.
 lane 1 NEB 2-log ladder, 2-4 pSB4A5, 5-7 R0040, 8-10 K123001, 11-13 B0014  lane 1 NEB 2-log ladder, 2-4 pNT003 insert, 5 R0010, 6 K123003, 7 B0014, 8 blank, 9-11 GFP insert, 12 GFP constitutive promoter, 13 GFP, 14 GFP terminator, 15 NEB 2-log ladder PCR concentrations
| Part |
Concentration (ng/ul) |
| R0040 for pNT002 |
56.4 |
| K123001 |
121.5 |
| B0014 for pNT002 |
100.8 |
| pSB4A5-1 |
98.0 |
| pSB4A5-2 |
97.4 |
|
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