Sunday, July 17

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Transformation of Dpn1-digested plasmid

1pm

Transformed contents of tubes 3a, 3b, 4a using transformation protocol with the following changes:

  • 2µl of mutagenesis mix used instead of 1µl.
  • LB media instead of SOC.

Timeline

  • Transformanda on ice at 1305h.
  • Heat shocked @ 1335h.
  • incubating at 37˚C until 1415.

Retrieved from "https://2011.igem.org/Team:Baltimore/Notebook/Transformation"

Team:Baltimore/Notebook

Hours

Retrieved from "http://2011.igem.org/Sunday,_July_17"