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From 2011.igem.org

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Contents 1 Abstract 2 Project Details 2.1 Part 2 2.2 The Experiments 2.3 Part 3 3 Results

Abstract

In the bodies of people with type one diabetes, the ability to both recognize and respond to glucose concentrations in the blood has been compromised. As a result, glucose accumulates to dangerous levels in the blood. High blood glucose concentrations can cause irreversible damage to several critical organs, impairing the functionality of those organs. With accessible parts from the iGEM registry we will attempt to integrate a glucose controlled promoter gene linked to a green fluorescence production gene and an insulin production gene. We will then determine the concentrations of glucose to which the promoter responds. Once the concentration is known, we will attempt to mutate the glucose controlled promoter gene so that it will respond to, and be activated by, concentrations of glucose closer to those of the average human (roughly 5mM).

Project Details

Part 2

The Experiments

Part 3

Results