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July 18
Colony PCR of colonies from positive Gibson plates from Friday, for vector and insert
Colony PCR of 16s plasmids, for vector and insert
Gel of short PCR parts to confirm they are being amplified
Pulsed field gel with a larger amount of soil sample DNA
Retransform pSB3K5 into Xl-10s
Results
 Colonies 1-6 from Gibson plates; 7 and 8 are reswabs of two colonies. From left to right: DNA ladder,(PNT001; colony 1; vector), P2v, P3v, P4v, P5v, P6v, P7v, P8v, (PNT001; colony 1; insert), P3i, P4i, P5i, P6i  from left to right: DNA ladder, (PNT001; reswab 7; insert), P8i, (16s; colony A; vector), 16Bv, 16Cv, (16s; A; insert), 16Bi, 16Ci
July 19
PCR parts for Gibson assembly of pNT001, pNT003 and Joe's positive GFP control
Results
pSB3K5 plate had 0 colonies.
PFGE of soil-extracted DNA shows a smear; will do gel extraction of ~40kbp sequences for fosmid kit.
Gibson PCR concentrations
| Part |
Concentration (ng/ul) |
| R0010 for pNT001 |
86.4 |
| B0014 for pNT001 |
128.3 |
| B0014 for pNT003 |
89.1 |
| promoter for GFP Gibson positive control |
51.9 |
| GFP for positive control |
130.4 |
| terminator for positive control |
82.5 |
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