Uppsala-Sweden/12 July 2011

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Welcome to Uppsala-SwedeniEM '2011

Notebook

June
M	T	W	T	F	S	S
		[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/1_June_2011&action=edit 1]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/2_June_2011&action=edit 2]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/3_June_2011&action=edit 3]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/4_June_2011&action=edit 4]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/5_June_2011&action=edit 5]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/6_June_2011&action=edit 6]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/7_June_2011&action=edit 7]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/8_June_2011&action=edit 8]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/9_June_2011&action=edit 9]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/10_June_2011&action=edit 10]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/11_June_2011&action=edit 11]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/12_June_2011&action=edit 12]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/13_June_2011&action=edit 13]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/14_June_2011&action=edit 14]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/15_June_2011&action=edit 15]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/16_June_2011&action=edit 16]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/17_June_2011&action=edit 17]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/18_June_2011&action=edit 18]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/19_June_2011&action=edit 19]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/20_June_2011&action=edit 20]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/21_June_2011&action=edit 21]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/22_June_2011&action=edit 22]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/23_June_2011&action=edit 23]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/24_June_2011&action=edit 24]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/25_June_2011&action=edit 25]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/26_June_2011&action=edit 26]
[http://2011.igem.org/Uppsala-Sweden/27_June_2011 27]	[http://2011.igem.org/Uppsala-Sweden/28_June_2011 28]	[http://2011.igem.org/Uppsala-Sweden/29_June_2011 29]	[http://2011.igem.org/Uppsala-Sweden/30_June_2011 30]

July
M	T	W	T	F	S	S
				[http://2011.igem.org/Uppsala-Sweden/1_July_2011 1]	[http://2011.igem.org/Uppsala-Sweden/2_July_2011 2]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/3_July_2011&action=edit 3]
[http://2011.igem.org/Uppsala-Sweden/4_July_2011 4]	[http://2011.igem.org/Uppsala-Sweden/5_July_2011 5]	[http://2011.igem.org/Uppsala-Sweden/6_July_2011 6]	[http://2011.igem.org/Uppsala-Sweden/7_July_2011 7]	[http://2011.igem.org/Uppsala-Sweden/8_July_2011 8]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/9_July_2011&action=edit 9]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/10_July_2011&action=edit 10]
[http://2011.igem.org/Uppsala-Sweden/11_July_2011 11]	[http://2011.igem.org/Uppsala-Sweden/12_July_2011 12]	[http://2011.igem.org/Uppsala-Sweden/13_July_2011 13]	[http://2011.igem.org/Uppsala-Sweden/14_July_2011 14]	[http://2011.igem.org/Uppsala-Sweden/15_July_2011 15]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/16_July_2011&action=edit 16]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/17_July_2011&action=edit 17]
[http://2011.igem.org/Uppsala-Sweden/18_July_2011 18]	[http://2011.igem.org/Uppsala-Sweden/19_July_2011 19]	[http://2011.igem.org/Uppsala-Sweden/20_July_2011 20]	[http://2011.igem.org/Uppsala-Sweden/21_July_2011 21]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/22_July_2011&action=edit 22]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/23_July_2011&action=edit 23]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/24_July_2011&action=edit 24]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/25_July_2011&action=edit 25]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/26_July_2011&action=edit 26]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/27_July_2011&action=edit 27]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/28_July_2011&action=edit 28]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/29_July_2011&action=edit 29]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/30_July_2011&action=edit 30]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/31_July_2011&action=edit 31]

August
M	T	W	T	F	S	S
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/1_August_2011&action=edit 1]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/2_August_2011&action=edit 2]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/3_August_2011&action=edit 3]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/4_August_2011&action=edit 4]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/5_August_2011&action=edit 5]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/6_August_2011&action=edit 6]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/7_August_2011&action=edit 7]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/8_August_2011&action=edit 8]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/9_August_2011&action=edit 9]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/10_August_2011&action=edit 10]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/11_August_2011&action=edit 11]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/12_August_2011&action=edit 12]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/13_August_2011&action=edit 13]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/14_August_2011&action=edit 14]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/15_August_2011&action=edit 15]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/16_August_2011&action=edit 16]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/17_August_2011&action=edit 17]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/18_August_2011&action=edit 18]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/19_August_2011&action=edit 19]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/20_August_2011&action=edit 20]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/21_August_2011&action=edit 21]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/22_August_2011&action=edit 22]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/23_August_2011&action=edit 23]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/24_August_2011&action=edit 24]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/25_August_2011&action=edit 25]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/26_August_2011&action=edit 26]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/27_August_2011&action=edit 27]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/28_August_2011&action=edit 28]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/29_August_2011&action=edit 29]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/30_August_2011&action=edit 30]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/31_August_2011&action=edit 31]

September
M	T	W	T	F	S	S
			[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/1_September_2011&action=edit 1]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/2_September_2011&action=edit 2]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/3_September_2011&action=edit 3]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/4_September_2011&action=edit 4]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/5_September_2011&action=edit 5]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/6_September_2011&action=edit 6]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/7_September_2011&action=edit 7]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/8_September_2011&action=edit 8]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/9_September_2011&action=edit 9]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/10_September_2011&action=edit 10]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/11_September_2011&action=edit 11]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/12_September_2011&action=edit 12]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/13_September_2011&action=edit 13]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/14_September_2011&action=edit 14]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/15_September_2011&action=edit 15]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/16_September_2011&action=edit 16]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/17_September_2011&action=edit 17]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/18_September_2011&action=edit 18]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/19_September_2011&action=edit 19]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/20_September_2011&action=edit 20]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/21_September_2011&action=edit 21]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/22_September_2011&action=edit 22]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/23_September_2011&action=edit 23]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/24_September_2011&action=edit 24]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/25_September_2011&action=edit 25]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/26_September_2011&action=edit 26]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/27_September_2011&action=edit 27]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/28_September_2011&action=edit 28]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/29_September_2011&action=edit 29]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/30_September_2011&action=edit 30]

October
M	T	W	T	F	S	S
					[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/1_October_2011&action=edit 1]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/2_October_2011&action=edit 2]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/3_October_2011&action=edit 3]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/4_October_2011&action=edit 4]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/5_October_2011&action=edit 5]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/6_October_2011&action=edit 6]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/7_October_2011&action=edit 7]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/8_October_2011&action=edit 8]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/9_October_2011&action=edit 9]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/10_October_2011&action=edit 10]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/11_October_2011&action=edit 11]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/12_October_2011&action=edit 12]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/13_October_2011&action=edit 13]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/14_October_2011&action=edit 14]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/15_October_2011&action=edit 15]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/16_October_2011&action=edit 16]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/17_October_2011&action=edit 17]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/18_October_2011&action=edit 18]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/19_October_2011&action=edit 19]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/20_October_2011&action=edit 20]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/21_October_2011&action=edit 21]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/22_October_2011&action=edit 22]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/23_October_2011&action=edit 23]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/24_October_2011&action=edit 24]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/25_October_2011&action=edit 25]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/26_October_2011&action=edit 26]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/27_October_2011&action=edit 27]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/28_October_2011&action=edit 28]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/29_October_2011&action=edit 29]	[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/30_October_2011&action=edit 30]
[http://2011.igem.org/wiki/index.php?title=Uppsala-Sweden/31_October_2011&action=edit 31]

2011-07-12

The day started by running a gel of ccaS mutant, amilCP-BB10, and amilGFP-PstI. At the same time we prepared the sample (ccaR, ccaS, RBS-ho1 and RBS-pcyA) for sequencing. Plating of terminator B1001, PLlacO, pSB3T5 and pSB4K5. PCR purification of ccaS, amilGFP, amilCP, clone amilCP and PcpcG2 (Purification protocol). Re-ligation and transform mutagenized ccaS and amilGFP. Transformation of amilCP, clone amilCP, TetR inverter and pcpcG2. Re-streak of cI inverter transformants from 2011-07-11.

The transformation of inverters from yesterday didn’t go well. The lambda-cI-inverter had only a few colonies, while the tetR inverter had no colonies at all. So TetR inverter has to be redone. This time the transformation used 2 µl of DNA from the MIT distribution.

Furthermore new plates containing all antibiotics and some containing teracyclin were made.