Team:Caltech/Week 6

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Caltech iGEM 2011



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July 18

Run colony PCR of positive Gibson plates from Friday
Run gel of short PCR parts to confirm they are being amplified
Run pulsed field gel with a larger amount of soil sample DNA
Retransform pSB3K5 into Xl-10s

Results

July 19

PCR parts for Gibson assembly of pNT001, pNT003 and Joe's positive GFP control

Results

pSB3K5 plate had 0 colonies.

Gibson PCR concentrations

Part Concentration (ng/ul)
R0010 for pNT001 86.4
B0014 for pNT001 128.3
B0014 for pNT003 89.1
promoter for GFP Gibson positive control 51.9
GFP for positive control 130.4
terminator for positive control 82.5


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