Uppsala-Sweden/27 June 2011

From 2011.igem.org

Revision as of 15:39, 20 July 2011 by Phanirkb (Talk | contribs)








2011-06-27

This week we started with testing the competence in our cells. For this transformation we used one positive control (plasmid pUC19 from New England Biolabs) as well as a negative control without plasmid. For the procedure we followed the protocol for transforming TOP10 competent cells. The result was good, we measured a competence efficiency of 1.7 * 10^8 transformants /ug DNA.

Started overnight cultures of E coli carrying the plasmids pGEM11- amilGFP (green output) and pGEM14- amilCP (blue output). For this procedure we followed the protocol Overnight culture and glycerol stock. The strains carrying the amilGFP and amilCP plasmids were provided by J.F Miller, UCLA.
Retrieved from "http://2011.igem.org/Uppsala-Sweden/27_June_2011"