July, 11th
| Plasmid |
Kind |
DNA (μl) |
H2O (μl) |
Enzyme 1 (μl) |
Enzyme 2 (μl) |
Buffer H (μl) |
Final Volume (μl) |
| E1-2 |
|
18 |
2.5 |
1 XbaI |
1 PstI |
2.5 |
25 |
| E2-2 |
|
18 |
2.5 |
1 XbaI |
1 PstI |
2.5 |
25 |
| E3-1 |
|
18 |
2.5 |
1 XbaI |
1 PstI |
2.5 |
25 |
| E4-2 |
|
18 |
2.5 |
1 XbaI |
1 PstI |
2.5 |
25 |
| E5-2 |
|
18 |
2.5 |
1 XbaI |
1 PstI |
2.5 |
25 |
| E6-1 |
|
11 |
9.5 |
1 XbaI |
1 PstI |
2.5 |
25 |
| E7-2 |
|
18 |
2.5 |
1 XbaI |
1 PstI |
2.5 |
25 |
| E8-3 |
|
20.5 |
0 |
1 SpeI |
1 PstI |
2.5 |
25 |
| <partinfo>BBa_R0040</partinfo> |
|
14.5 |
6 |
1 SpeI |
1 PstI |
2.5 |
25 |
| <partinfo>BBa_C0261</partinfo> |
|
18 |
2.5 |
1 XbaI |
1 PstI |
2.5 |
25 |
| <partinfo>BBa_I13507</partinfo> |
|
10 |
10.5 |
1 XbaI |
1 PstI |
2.5 |
25 |
| <partinfo>BBa_B0034</partinfo> |
|
10 |
10.5 |
1 SpeI |
1 PstI |
2.5 |
25 |
Reactions were incubated at 37°C for three hours while two medium-size agarose gels were prepared according to protocols.
In the afternoon gel electrophoresis was performed.
link alle 2 img dei gel con ling wiki
As shown, in figure all clones were positive, so we cut and purified the bands of interest.
After gel extraction, cut DNA was quantified:
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