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Team:UNIPV-Pavia/Calendar/July/settimana3
From 2011.igem.org
Revision as of 21:35, 19 July 2011 by
Tommy Goggia
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UNIPV TEAM 2011
Project
Background & Motivation
Solution
Modelling
Results
References
Lab
Materials & Methods
Protocols
Instruments
Measurements
Notebook
Calendar
Freezer Management
Biosafety
Parts
Submitted
Characterized
Data Page
About us
Team
Pavia
Gallery
Sponsors
March
M
T
W
T
F
S
S
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
April
M
T
W
T
F
S
S
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
May
M
T
W
T
F
S
S
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
June
M
T
W
T
F
S
S
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
July
M
T
W
T
F
S
S
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
August
M
T
W
T
F
S
S
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
September
M
T
W
T
F
S
S
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
October
M
T
W
T
F
S
S
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
JULY: WEEK 3
July, 11th
Plasmid
Kind
DNA (μl)
H
2
O (μl)
Enzyme 1 (μl)
Enzyme 2 (μl)
Buffer H (μl)
Final Volume (μl)
E1-2
18
2.5
1 XbaI
1 PstI
2.5
25
E2-2
18
2.5
1 XbaI
1 PstI
2.5
25
E3-1
18
2.5
1 EcoRI
1 SpeI
2.5
25
E4-2
18
2.5
1 XbaI
1 PstI
2.5
25
E5-2
18
2.5
1 XbaI
1 PstI
2.5
25
E6-1
11
9.5
1 XbaI
1 PstI
2.5
25
E7-2
18
2.5
1 XbaI
1 PstI
2.5
25
E8-3
20.5
0
1 SpeI
1 PstI
2.5
25
<partinfo>BBa_R0040</partinfo>
14.5
6
1 SpeI
1 PstI
2.5
25
<partinfo>BBa_C0261</partinfo>
18
2.5
1 XbaI
1 PstI
2.5
25
<partinfo>BBa_I13507</partinfo>
10
10.5
1 XbaI
1 PstI
2.5
25
<partinfo>BBa_B0034</partinfo>
10
10.5
1 SpeI
1 PstI
2.5
25
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