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From 2011.igem.org

Contents 1 July 04 2011 1.1 Week of Jun27 - Jul04 1.1.1 IDI1, HMG2, erg20-2 1.1.2 1,8-Cineole 1.1.3 beta-pinene

July 04 2011

Week of Jun27 - Jul04

IDI1, HMG2, erg20-2

• Validated competent cells by transforming with pet100 vector containing Cineole synthase. They are in working order.
• Amplified 5 plasmids received from Greece (IDI1, HMG2, p300, Cin, Sab). NOTE: did not properly centrifuge during DNA precipitation stage. Will validate plasmids in yeast transformations to make sure plasmids are in working order.
• Awaiting materials from France (erg20-2).

1,8-Cineole

• Ran a gel to test restriction digest and variant SDM-PCR product.

beta-pinene

• SDM beta-pinene again by using both Pfu and Taq.
• Gel verification shows a smear.
• Troubleshooting: run a gel on the miniprep of the beta-pinene plasmid received from Rafael to make sure that the DNA hasn't been contaminated/digested.