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From 2011.igem.org

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May 31

• Preliminary Shopping List

1. 1. PCR buffer w/o MgCl - 5ML - $45.00
   2. M8787- 5ML -MgCl Reagent- $49.30
   3. D7295-.5ML - dNTP - $73.00
   4. D4184-250UN - Taq Polymerase - $150.00
   5. D8045-250UN - AccuTaq - $300
   6. E1385-5ML – Ethidium Bromide – $23.70
   7. T8280-1L – Tris Acetate EDTA Buffer – $45.10
   8. NA1020-1KT – PCR Clean-Up Kit -$102.50
   9. NA1111-1KT - Gel Extraction Kit - $102.00

Subtotal: $890.60

• Met with Cohen Lab grad students to finalize plan and get cassettes.
• Received Leu2 cassette in pRS305 plasmid.
• Received Ura3 cassette in pRS306 plasmid.

June 1

• Lesson from Bert Berla on how to design primers
• Codon Optimized the four genes using tool at: http://www.encorbio.com/protocols/Codon.htm
  • Double checked gene and restriction sites to make sure enzyme does not cut in the middle of the gene
• Design Primers for cassettes
  • Ura3, Leu2, KanMX4, and NatMX4