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Team:Freiburg/Notebook/13 July
From 2011.igem.org
Contents |
red light receptor
Investigators: Julia
Waiting for sequencing results of parts BBa_I15008 and BBa_I15009. Julia did Minipreps of single colonies with PCR-ampilfied Chp8 in pSB1T3. She also did a test digest to see if the insert is in the vector.
Miniprep
Investigators: Jakob
Miniprep of cph8 from M30109 in pSB1T3 DNA concentration was measured with NanoDrop:
| samples | DNA concentration | 260/280 | 230 |
| cph8a | 33.7 | 1.79 | 1.74 |
| cph8b | 41.2 | 1.79 | 1.39 |
| cph8c | 24.1 | 1.87 | 1.47 |
| cph8d | 30 | 1.74 | 1.84 |
| cph8e | 34 | 1.81 | 1.86 |
| cph8f | 30 | 1.83 | 1.81 |
Testdigest
Investigators: Julia
Testdigest of cph8
- Enzyme 1: EcoRI
- Enzyme 2: PstI
Transformation
Investigators: Julia
Transformation of BBa_I15010 (in the vector pSB2K3) in cells. Sophie plated out the cells.
Comments: No colonies grew on the plates.
PCR
Investigators: Julia
New PCR for red light receptor.
Primer:
- P10
- P11
DNA template:
- M30109
Precipitator
Miniprep
Investigators: Sophie, Sandra
Comments: Ligation, transformation and picking of the clones was done before the miniprep was performed.
Miniprep of the plastik-binding domain: There were always picked two colonies of the same plate (sample and sample*).
| sample / sample* | DNA concentration | 280/230 | 260/230 |
| M2a alpha / M2a* alpha | 66.5 / 91.6 | 1.84 / 1.75 | 1.76 / 1.40 |
| M2a beta / M2a* beta | 83.8 / 13.8 | 1.80 / 1.72 | 1.52 / 0.97 |
| M2a gamma / M2a* gamma | 118.9 / 56.1 | 1.68 / 1.86 | 1.00 / 2.13 |
| M2a delta / M2a* delta | 101.5 / 66.0 | 1.74 / 1.85 | 1.26 / 2.16 |
| M2a epsilon / M2a* epsilon | 112.9 / 69.9 | 1.68 / 1.82 | 1.00 / 1.82 |
| M2b alpha / M2a* alpha | 79.7 / 74.9 | 1.87 / 1.83 | 2.20 / 2.14 |
| M2b Beta/M2b* beta | 122.3/74.4 | 1.71/1.84 | 1.04/2.18 |
| M2b gamma / M2b* gamma | 111.2 / 72.0 | 1.70 / 1.83 | 1.08 / 1.99 |
| M2b epsilon / M2b* epsilon | 88.5 / 90.09 | 1.72 / 1.79 | 1.19 / 1.78 |
| M3 alpha / M3* alpha | 136.3 / 93.9 | 1.71 / 1.78 | 1.10 / 1.74 |
| M3 beta / M3* beta | 54.7 / 79.2 | 1.91 / 1.83 | 2.03 / 2.13 |
| M3 gamma / M3* gamma | 130.6 / 141.7 | 1.66 / 1.80 | 0.87 / 1.88 |
| M3 delta / M3* delta | 79.6 / 82.9 | 1.76 / 1.85 | 1.27 / 2.19 |
| M3 epsilon / M3* epsilon | 84.1 / 122.5 | 1.79 / 1.69 | 1.45 / 1.03 |
