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From 2011.igem.org

Contents 1 red light receptor 1.1 Miniprep 1.2 Testdigest 2 Precipitator 2.1 Miniprep

red light receptor

Investigators: Julia

Waiting for sequencing results of parts BBa_I15008 and BBa_I15009. Julia did Minipreps of single colonies with PCR-ampilfied Chp8 in pSB1T3. She also did a test digest to see if the insert is in the vector.

Miniprep

Investigators: Jakob

Miniprep of cph8 from M30109 in pSB1T3 DNA concentration was measured with NanoDrop:

samples	DNA concentration	260/280	230
cph8a	33.7	1.79	1.74
cph8b	41.2	1.79	1.39
cph8c	24.1	1.87	1.47
cph8d	30	1.74	1.84
cph8e	34	1.81	1.86
cph8f	30	1.83	1.81

Testdigest

Investigators: Julia

Testdigest of cph8

• Enzyme 1: EcoRI
• Enzyme 2: PstI

Precipitator

Miniprep

Investigators: Sophie, Sandra

Comments: Ligation, transformation and picking of the clones was done before the miniprep was performed.

Miniprep of the plastik-binding domain: There were always picked two colonies of the same plate (sample and sample*).

sample / sample*	DNA concentration	280/230	260/230
M2a alpha / M2a* alpha	66.5 / 91.6	1.84 / 1.75	1.76 / 1.40
M2a beta / M2a* beta	83.8 / 13.8	1.80 / 1.72	1.52 / 0.97
M2a gamma / M2a* gamma	118.9 / 56.1	1.68 / 1.86	1.00 / 2.13
M2a delta / M2a* delta	101.5 / 66.0	1.74 / 1.85	1.26 / 2.16
M2a epsilon / M2a* epsilon	112.9 / 69.9	1.68 / 1.82	1.00 / 1.82
M2b alpha / M2a* alpha	79.7 / 74.9	1.87 / 1.83	2.20 / 2.14
M2b Beta/M2b* beta	122.3/74.4	1.71/1.84	1.04/2.18
M2b gamma / M2b* gamma	111.2 / 72.0	1.70 / 1.83	1.08 / 1.99
M2b epsilon / M2b* epsilon	88.5 / 90.09	1.72 / 1.79	1.19 / 1.78
M3 alpha / M3* alpha	136.3 / 93.9	1.71 / 1.78	1.10 / 1.74
M3 beta / M3* beta	54.7 / 79.2	1.91 / 1.83	2.03 / 2.13
M3 gamma / M3* gamma	130.6 / 141.7	1.66 / 1.80	0.87 / 1.88
M3 delta / M3* delta	79.6 / 82.9	1.76 / 1.85	1.27 / 2.19
M3 epsilon / M3* epsilon	84.1 / 122.5	1.79 / 1.69	1.45 / 1.03