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Team:Freiburg/Notebook/13 July
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Contents |
red light receptor
Investigators: Julia
Waiting for sequencing results of parts BBa_I15008 and BBa_I15009. Julia did Minipreps of single colonies with PCR-ampilfied Chp8 in pSB1T3. She also did a test digest to see if the insert is in the vector.
Miniprep
Investigators: Jakob
Miniprep of cph8 from M30109 in pSB1T3 DNA concentration was measured with NanoDrop:
| samples | DNA concentration | 260/280 | 230 |
| cph8a | 33.7 | 1.79 | 1.74 |
| cph8b | 41.2 | 1.79 | 1.39 |
| cph8c | 24.1 | 1.87 | 1.47 |
| cph8d | 30 | 1.74 | 1.84 |
| cph8e | 34 | 1.81 | 1.86 |
| cph8f | 30 | 1.83 | 1.81 |
Testdigest
Investigators: Julia
Testdigest of cph8
- Enzyme 1: EcoRI
- Enzyme 2: PstI
Precipitator
Miniprep
Investigators: Sophie, Sandra
Comments: Ligation, transformation and picking of the clones was done before the miniprep was performed.
Miniprep of the plastik-binding domain:
| samples | DNA concentration | 280/230 | 260/230 |
| M2a alpha/M2a* alpha | 66.5/91.6 | 1.84/1.75 | 1.76/1.40 |
| M2a beta/M2a* beta | 83.8/13.8 | 1.80/1.72 | 1.52/0.97 |
| M2a gamma/M2a* gamma | 118.9/56.1 | 1.68/1.86 | 1.00/2.13 |
| M2a delta/M2a* delta | 101.5/66.0 | 1.74/1.85 | 1.26/2.16 |
| M2a epsilon/M2a* epsilon | 112.9/69.9 | 1.68/1.82 | 1.00/1.82 |
| M2b alpha/M2a* alpha | 79.7/74.9 | 1.87/1.83 | 2.20/2.14 |
| M2b Beta/M2b* beta | 122.3/74.4 | 1.71/1.84 | 1.04/2.18 |
| M2b gamma/M2b* gamma | 111.2/72.0 | 1.70/1.83 | 1.08/1.99 |
| M2b epsilon/M2b* epsilon | 88.5/90.09 | 1.72/1.79 | 1.19/1.78 |
| M3 alpha/M3* alpha | 136.3/93.9 | 1.71/1.78 | 1.10/1.74 |
| M3 beta/M3* beta | 54.7/79.2 | 1.91/1.83 | 2.03/2.13 |
| M3 gamma/M3* gamma | 130.6/141.7 | 1.66/1.80 | 0.87/1.88 |
| M3 delta/M3* delta | 79.6/82.9 | 1.76/1.85 | 1.27/2.19 |
| M3 epsilon/M3* epsilon | 84.1/122.5 | 1.79/1.69 | 1.45/1.03 |
