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From 2011.igem.org

Contents 1 Sequence Analysis 1.1 Lysis cassette 1.1.1 Lysis cassette and heat inducible promotor 1.2 green light receptor 1.2.1 CcaR 1.3 red light receptor 1.3.1 pycA+terminator 1.3.2 cph8 1.4 blue light receptor 1.4.1 LOV1 1.4.2 LOV3 2 Experiments 2.1 green light receptor 2.1.1 Gel 2.2 red light receptor 2.2.1 Gel 2.3 Precipitator 2.3.1 Plastic binding site

Sequence Analysis

Lysis cassette

Lysis cassette and heat inducible promotor

S15b: (Lysis cassette K124014) is named K124017 in the registry Part without RBS at the beginning Part without antiholin the sequece is also mutated leading to wrong amino acids if combined with RBS there would be too many bases (8) between RBS and coding sequence part is likely to be not effective

green light receptor

CcaR

S17: (CcaR with Prefix and RBS) is ok. Check RBS. S18: seems to be ok too.

red light receptor

pycA+terminator

S24: prefix and suffix not complete

cph8

S29: Sequence without part. Has to be done again.

blue light receptor

LOV1

S33: LOVtap only

LOV3

S35: is K322999. This sequence is the LOVtap with the tryptophane promoter, RBS, mRFP and two terminators.

Experiments

green light receptor

Gel

Investigators: Jakob

Comments: Miniprep from June,12 were loaded onto a gel.

Ccas (M31, M32, M30)in pSB1C3

Image of the gel of M30-MM32 and M36-M38

Comments: All the samples were negative.

red light receptor

Gel

Investigators: Jakob

Comments: Miniprep from June,12 were loaded onto a gel. If they are positive: Sequencing

ho1+terminator (M36, M37, M38) in pSB1C3

Comments: All the samples were negative. See image above.

Precipitator

Plastic binding site

Investigators: Sophie

Picking colonies (M2a, M2b, M3), inoculating LB (Cm) and let grow overnight. Start: 17:15