Team:Freiburg/Notebook/29 June

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Lysis cassette

3A-assembly: Digestion

Investigators: Sophie

The terminator (S21) was first digested with pycA (S23)

S21:

  • Enzyme 1: PstI
  • Enzyme 2: XbaI

S23:

  • Enzyme 1: EcorI
  • Enzyme 2: SpeI