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From 2011.igem.org

Contents 1 Meeting 1.1 green light receptor 1.2 blue light receptor 1.3 red light receptor 1.4 Lysis cassette 1.5 Precipitator 2 green light receptor 2.1 NAME OF YOUR EXPERIMENT 3 blue light receptor 3.1 NAME OF YOUR EXPERIMENT 4 red light receptor 4.1 NAME OF YOUR EXPERIMENT 5 Lysis cassette 5.1 NAME OF YOUR EXPERIMENT 6 Precipitator 6.1 NAME OF YOUR EXPERIMENT

Meeting

attendants: Tobi, Theo, Julia, Rüdiger, Jakob, Sandra

green light receptor

already done:

To-do:

blue light receptor

already done:

1. Transformation of Lov-Tap in cells.

To-do:

1. Cloning of promotor (medium) in Lov-Tap part and perform a transformation. Plating out of the cells on trypthophan-free medium.

red light receptor

already done:

To-do:

Lysis cassette

already done:

1. repressor part (BBa_K098995) has no bases between RBS (B0034) and start codon (K098997), resulting in no translation.
   • quickchange of the repressor part to insert 6bp between RBS and start codon

To-do:

1. DpnI digestion to digest the DNA template (methylated DNA) of the PCR to have only the new synthesized DNA strand.
   • After digestion with DpnI, transformation of cells with the corrected part (BBa_K08995)

Precipitator

already done:

To-do:

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME

Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME