Team:Cambridge/Protocols/DNA Precipitation

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Rescue precipitation of DNA

Method for recovering DNA after premature elution whilst following Gel Extraction of DNA.

Theory

Addition of positive sodium ions mitigates repulsive interactions of the negatively charged DNA backbone, allowing DNA to coelesce. In the presence of positive ions DNA is insoluble in alcohols, so will precipitate.

Practice

Safety

The safety implication of the procedure.