Team:CBNU-Korea

Synthetic minimal genome is the smallest possible group of genes that would be sufficient to sustain a cellular life form under the most favorable conditions imaginable that working like natural chromosome, bacteria have.

For synthesizing minimal genome, there are many things that we have to consider such as kind of genes, gene arrangement, direction of genes, codon usage, genome structure, stability and so on. Therefore it is very difficult to synthesize minimal genome considering ALL of these.

So we synthesize simple minimal chromosome employing V.cholerae O1 biovar eltor str. N16961chromosome II replication and partition system as the first step of synthetic minimal genome.

Also, we re-build essential gene database from DEG and NCBI. And using this database, we build genome designer which is based on essential gene database that is produced by sequential analysis process.