Team:Paris Liliane Bettencourt/Notebook/2011/07/13/

From 2011.igem.org

Revision as of 15:21, 14 July 2011 by Kyauy (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Kevin

Glycerol from lacO/tetO plasmids :

  • Centrifugation 10min - 4000 RPM
  • Put liquid on trash and vortex it
  • Resuspend with LB + Glycerol 20% (3mL on each tube)
  • Put it on cryovial tube (1,5mL on each tube -20°C / -80°C)

Cultur of glycerols :

10mL LB + 10μL Antibiotics (List on 2011/07/13)

Retrieved from "http://2011.igem.org/Team:Paris_Liliane_Bettencourt/Notebook/2011/07/13/"