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Team:Freiburg/Notebook/28 June
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21. Labday
3A assembly:Digestion, Ligation and Transformation for the receptor
Investigators: Julia
Digestion of ho1, pycA (chromophor) and terminator (pSB1AK3).
Ho1 and pycA:
Enzyme 1: EcoRI
Enzyme 2: SpeI
Terminator part (pSB1AK3):
Enzyme 1: EcoRI
Enzyme 2: XbaI
ligated vector was then transformed in cells and plated out on chloramphenicol plates.
PCR of GFP fused to plastic-binding domain
Investigators: Sophie, Rüdiger
S 14: GFP
Primer: GFPxb1Iup (P1)
GFPpbdSpedw (P3)
Comments: P1 did not work
