Team:Freiburg/Notebook/28 June

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21. Labday


3A assembly:Digestion and Ligation and Transformation

Investigators: Julia

Digestion of ho1, pycA and terminator (pSB1AK3)

Ho1 and pycA:
Enzyme 1: EcoRI
Enzyme 2: SpeI

Terminator part (pSB1AK3):
Enzyme 1: EcoRI
Enzyme 2: XbaI