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From 2011.igem.org

Contents 1 Sequence Analysis 1.1 Lysis cassette and heat induced promoter 1.1.1 Lysis cassette 1.2 Green light receptor 1.2.1 CcaR 1.2.2 pycA+terminator 1.2.3 cph8 1.2.4 LOV1 1.2.5 LOV3

Sequence Analysis

Lysis cassette and heat induced promoter

Lysis cassette

S15b: (Lysis cassette K124014) is named K124017 in the registry Part without RBS at the beginning Part without antiholin the sequece is also mutated leading to wrong amino acids if combined with RBS there would be too many bases (8) between RBS and coding sequence part is likely to be not effective

Green light receptor

CcaR

S17: (CcaR with Prefix and RBS) is ok. Check RBS. S18: seems to be ok too.

pycA+terminator

S24: prefix and suffix not complete

cph8

S29: Sequence without part. Has to be done again.

LOV1

S33: LOVtap only

LOV3

S35: is K322999. This sequence is the LOVtap with the tryptophane promoter, RBS, mRFP and two terminators.