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From 2011.igem.org

Contents 1 Sequence Analysis 1.1 Lysis cassette 1.1.1 S15b 1.2 Green light receptor 1.2.1 CcaR

Sequence Analysis

Lysis cassette

S15b

Lysis cassette K124014, named as K124017 Part without RBS at the beginning Part without antiholin the sequece is also mutated leading to wrong amino acids if combined with RBS there would be too many bases (8) between RBS and coding sequence part is likely to be not effective

Green light receptor

CcaR

S17 (CcaR with Prefix and RBS) is ok. Check RBS. S18 seems to be ok too.