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From 2011.igem.org

Monday

Lab Work

• 2 overnight cultures of A2 was put in a shaking cabinet in 37 degrees

• Biobrick A1 og A2 was mutated (again with template amounts 10,25 and 50 nm) and XL1-Blue competent cells were transformed

• We extended our cyp B1 with prefix and suffix by a PCR PCR approach.

The DNA obtained was purified by using a PCR purification kit, followed by gel purification and then extracted the DNA from the gel. The B1 DNA is now ready to be transfected into expression vectors.