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Team:Freiburg/Notebook/15 June
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??.Labday
PCR for Ccas and CcaR
Investigators: Julia
Ccas: cyanobacteriochrome is a green light receptor that induces the expression of pycobillisome linker protein
Ccar: cognate response regulator
Primer diluted, PCR for CcaS with P(rimer) 5, P7 and CcaR with P4, P6
32,5 µl H2O
2µl Primer fw
2µl Primer rev
1µl DNA ( synechocystis from Hess-Lab)
1µl dNTPs
0,5 µl Phusion
Miniprep
Investigators: Julia, Sandra
Comments: Miniprep from june,14 was showed bad results. Grew over night at 37°C.
Miniprep PR4a, PR4b, PR8a, PR8b, PR9a, PR9b
measurement of nanodrop was ok
