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Team:Nevada/Notebook/Temp
From 2011.igem.org
Contents |
Temp Calender
Week 1 - June 1st-5th
E. Coli
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Cyano
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Hexokinase coupled assay used to quantitate amounts of cyanobacteria glucose secretion.
* Rxn: 1. glucose + ATP --Hx-> G-6-P + ADP * 2. G-6-P + NAD+ --G-6-PDeH-> G-6-P + NADH
Reaction used Glucose assay kit (Genzyme) containing 2000U/L Hexokinase reagent and 4000U/L G-6-P DeH and measured glucose concentrations ranging from 0.05mM-0.25mM and absorbances were measured at 340.0 nm.
Results: Final NADH concentrations were determined using the molar extinction coefficient and were proportionate to initial glucose concentrations. Discussion: Focus is now to measure not only glucose, but fructose concentrations as well as both with be secreted in the form of sucrose using a D-glucose/D-fructose assay (Megaenzyme.
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