Team:Paris Liliane Bettencourt/Notebook/2011/07/05/

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ligation and transformation

ligation

BBa_k143079 6.4ng/µL in BBa_K090403 (vector) 9.2ng/µL
BBa_K145001 8.5ng/µL - BBa_B0015 (in the vector) 10.9ng/µL

Babak

Gel purification

well 1: ladder 100pb
from well 2: BBa_B1010 cut by EcoR1 and S, concentration of DNA=8,9ng/µL

Digestion

BBa_K090403 cut by EcoR1 and XbaI
BBa_K143079 cut by EcoR1 and SpeI
BBa_B0015 cut by EcoR1 and XbaI
BBa_E1010 cut by EcoR1 and SpeI
B4 pVeg + SpoVg,117pb, cut by EcoR1 and SpeI

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