Copenhagen/6 July 2011

From 2011.igem.org

Wednesday

Today we will make our first biobrick with the CYP 79A1 and finish the mutagenesis started yesterday.

Lab Work

• Digestion of our PCR products - see protocol Mutations

• Transformation of E.coli cells with the mutant plasmids - see protocol Transformations

• PCR on the purified mutant 79A1 CYP. By using our designed primers this will insert the suffix and prefix at each end of the CYP79A1, thus making it a biobrick.

Other Work

• We have send out e-mails to danish biotech companies in hope to achieve economical support.

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