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From 2011.igem.org

Minipreps, digestion, purification and ligation

Axel

Minipreps

• on BBa_K090403 (vector), concentration of DNA=248.8ng/µL
• on BBa_K143079 (device with constitutive promoter and GFP), concentration of DNA=500.2ng/µL
• on BBa_B0015, concentration of DNA=408.1ng/µL
• on BBa_E1010 concentration of DNA=330.8ng/µL
• on BBa_K145001, concentration of DNA=572.8ng/µL

10 µL of minipreped DNA corresponds to approximately 125ng of DNA (see fluorescence of the 3Kb band of the ladder)

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digestion

• BBa_K090403 cut by EcoR1 and XbaI
• BBa_K143079 cut by EcoR1 and SpeI
• BBa_B0015 cut by EcoR1 and XbaI
• BBa_E1010 cut by EcoR1 and SpeI
• BBa_K145001 cut by EcoR1 and SpeI

gel electrophoresis at 0.6% of Agarose

• well 1: ladder 1kb
• well 2: BBa_B0015 cut by EcoR1 and XbaI
• well 3: BBa_E1010 cut by EcoR1 and SpeI
• well 4: BBa_K145001 cut by EcoR1 and SpeI
• well 5: BBa_K090403 cut by EcoR1 and XbaI
• well 6: BBa_K143033 cut by EcoR1 and SpeI