Team:British Columbia/Notebook/4 July 2011

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Contents

July 04 2011

Week of Jun27 - Jul04

IDI1, HMG2, erg20-2

  • Validated competent cells by transforming with pet100 vector containing Cineole synthase. They are in working order.
  • Amplified 5 plasmids received from Greece (IDI1, HMG2, p300, Cin, Sab). NOTE: did not properly centrifuge during DNA precipitation stage. Will validate plasmids in yeast transformations to make sure plasmids are in working order.
  • Awaiting materials from France (erg20-2).

1,8-Cineole

  • Ran a gel to test restriction digest and variant SDM-PCR product.
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