Modular GPCR Constructs
In approaching our chemotaxis project, several G-protein coupled receptors were cultivated from multiple mammalian sources.
BBa_K631039 - nahD: The coding sequence for the nahD protein, from P. peutida, capable of degrading napthalene.
BBa_K631005 - NPY1R_Mus:The coding sequence for the NPY1R protein. This is a GPCR from Mus musculus.
BBa_K631006 - NPY1R_Rat:The coding sequence for the NPY1R protein from Rattus norvegius. This is also a GPCR .
BBa_K631007 - ADBR2: The coding sequence for ADBR2, a mouse GPCR that binds phenol (albuteroll). Length: 1257
BBa_K631009 - OX1R: Coding sequence for OX1R, a mouse GPCR that binds cholorobenzoic acid.
BBa_K631010 - MARM: Coding sequence for MARM1, a rat receptor that binds phenol (hydroquinone).
BBa_K631013 - RFP: Red fluorescent protein for the reporter system in our worm.
BBa_K631036 - eCFP:UNC-54: Eukaryotic cyano-fluorescent protein for the reporter system in our worm. Length: 1629.
Regulatory Constructs for C. elegans
BBa_K631035 - Str2: A promoter for AWC neurons, normally used for a 7-transmembrane olfactory receptor. We can use this as a promoter to integrate non-native GPCRs into the AWC neuron membrane.
BBa_K631001 - ODR-7: A promoter for a 7-transmembrane olfactory receptor in AWA olfactory neurons. This can be used for integrating non-native GPCRs. Length: 1503
BBa_K631002 - let-858: A promoter for a protein essential for the development of the worm. We used this in one of our composite parts to express fluorescence in the worm.
BBa_K631003 - GPC-1: A promoter for one of the gamma sub-units of the GPCR binding proteins. We used this for our fluorescence as well. Length: 2000
BBa_K631004 - GCY7: A promoter for a membrane guanylyl cyclase protein. This can be used for a non-native receptor or fluorescence reporter.
Composite Constructs
BBa_K631031 - ODR7:OX1R:UNC-54: A composite part designed to place the non-native OX1R into the neurons of the worm using the ODR-7 promoter for the ability to detect cholorobenzoic acid.
BBa_K631032 - ODR7:ADBR2:UNC-54: A composite part designed to place the non-native ADBR2 into the neurons of the worm using the ODR-7 promoter for the ability to detect phenol (albuteroll). Length: 3666
BBa_K631033 - GCY7:ADBR2:UNC-54: Another composite part with ADBR2, under the GCY7 promoter.
BBa_K631037 - GPC-1:eCFP:UNC-54: This is a composite part for our reporter system using cyano-fluorescent protein under the GPC-1 promoter. Length: 3787
BBa_K631034 - let858:RFP:UNC-54:This is a composite part for our reporter system using red fluorescent protein under the let858 promoter.
BBa_K631038 - GCY-7:eCFP:UNC-54: This is a composite part for our reporter system using cyano-fluorescent protein under the GCY-7 promoter.
BBa_K631039 - nahD: The coding sequence for the nahD protein, from pseudomonas peutida, capable of degrading napthalene. Expressing this in our worm may give it the ability to degrade naphthalene.
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