From Monday the 10th of October to Sunday the 16th of October 2011

Monday

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Strain construction

We're back to the lab for few more experiments, so we're building up again the strain we'll need for these experiments.

Transformations : PHL1414/pIG2, PHL1414/pIG6, PHL1414/pIG16, MC4100/p85, MC4100/pIG2, MC4100/pIG6
Note:There was less than 2µl of p85.
Dickeya strain A350 was transformed by electroporation with pIG6 and pIG2. A 5ml of LB liquid culture of 1 clone of both strain is started.
Do not forget that Dickeya doesn't like the cold, so keep the Petri dishes on the bench.

Tuesday

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Strain construction

Transformations results:
MC4100/pIG6 --> 1 clone
MC4100/pIG2 --> 2 clones
MC4100/p85 --> 0 clone
PHL1414/pIG2 --> 0 clone
PHL1414/pIG16 --> 5 clones
A 5ml liquid culture of each clone obtened is started.
Extraction and digestion by E and P.
The gel electrophoresis shows that the clones of MC4100/pIG2, MC4100/pIG6, PHL1414/pI16, PHL1414/pIG6 and A350/pIG2 are ok. There is nothing on the digested A350/pIG6 track.

New test for the transformation of pIG20 (same as pIG2) in PHL1414.

Strain collection

S = A350/pIG6
S = A350/pIG2
The final concentration of glycerol is 40%. (500µl of culture + 500µl of Y80%)