| Day |
Note |
| Sept.26th Monday |
•Miniprep: Rush-3,-4,-12
•Run the gel of digestion test results:
RFP+YFP+1K3-7,-16 |
•PCR amplify: Rush-3,-4,-12
•Sample and test the intensity of fluorescence once or twice an hour during the growth of E.coli |
|
| Sept.27th Tuesday |
•Ligate and transform:
RFP+YFP+CFP+1C3 RFP+YFP+1C3 |
•PCR amplify:20H
•Cut: Rush Bone,22B |
|
| Sept.28th Wednesday |
•Cultivate: DH5αwith Device CFP,RFP,YFP and without any device
•Ligate and purify:Rush+22B
•Cut and purify:
the product of the ligation |
•Run the gel: Digested Rush and 22B,Lig1
•Self-ligate:Digested Lig
•Colony PCR: RFP+YFP+CFP+1C3
•Cut and purify: RFP+YFP+1K3,12I |
|
| Sept.29th Thursday |
•Ligate and transform: RFP+YFP+1K3&1C3,
RFP+YFP&1C3
•Colony PCR:RFP+YFP+CFP+1C3
•Preserve the colony on the plate |
|
•Plate the ligation results: RFP+1C3,
RFP+YFP+CFP+1C3
Colony PCR:RFP+YFP+1C3
RFP+YFP+CFP+1C3
•Cultivate the above
|
|
| Sept.30th Friday |
|
| Oct.1st Saturday |
•Colony PCR: Failed
•MIniprep: 10I |
•Cut: 10I+22B,20J,1T3
•Ligate:10I+22B+1T3 |
•Cultivate:2I,2M |
|
| Oct.2nd Sunday |
•Transform the ligation results:
22B+10I(1T3),20J+10I(1T3)
•Miniprep and Preserve:2M,2I |
•Cut:fdhf,Vgb,2M,2I
•Purify:RFP+YFP+CFP+1C3 |
•Ligate and transform:
RFP+YFP+CFP&1A3k
fdhf+2M&1K3,Vgb+2I&1K3 |
|
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