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Team:Paris Bettencourt/Experiments/Methodologies/Integration
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Integration plasmid
In parallel to the transformation with the replicative plasmid (pHM3), we tried to use a Bacillus subtilis integration plasmid. For that, we used the construction of the B.subtilis integration vector pSBINT1 built by the 2008 Cambridge iGEM team.
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At first, we managed to clone each of our parts in the 5' integration sequence for the AmyE locus of B.subtilis (<partinfo>K143001</partinfo>).
After, we want to clone them into the integrative plasmid (<partinfo>K090403</partinfo>).
