Data Page

Figure 1: Two variants of a dual color inducible gene network integrated into the Ade2 and Ade4 loci of S. cerevisiae. A – BBa_K642000 (which is tagged with BFP) binds to its GFP tagged trans-regulated promoter blocking transcription. B – BBa_K642004 (which is tagged with BFP and a VP16 activation domain) binds to its GFP tagged trans-regulated promoter activating transcription.

Data for our new favorite characterized parts:

1. http://partsregistry.org/wiki/index.php?title=Part:BBa_K642000 – BBa_K642000, TetR repressor tagged with yeast codon optimized BFP: when placed downstream of the Gal1/10 promoter, can be induced using galactose to produce BFP expression. Our goal is to eventually have this tagged repressor act on the TX proximal promoter fused to GFP but we have not completed this.
2. http://partsregistry.org/wiki/index.php?title=Part:BBa_K642004 – BBa_K642004, TetR repressor tagged with yeast codon optimized BFP and a VP16 activation domain: when placed downstream of the Gal1/10 promoter, can be induced using galactose to produce BFP expression. Our goal is to eventually have this tagged repressor act on a tet responsive element upstream of the transcription start site of GFP but we have not completed this.
3. http://partsregistry.org/Part:BBa_K642007 - BBa_K642007, Constitutive Adh1 distal-Gal1 proximal promoter tagged with yeGFP: