We set out to develop a quick, easy process for the expansion of basic parts into a part families. Our method employs a simplemutagenic PCR protocol that uses standard VF2 and VR primers and materials most iGEM teams already have in their labs. We chose to prototype this process by creating a part family from the LacI promoter R0010, and to mutate GFP to visually assess our ability to create functional protein mutants.

As of November 2011, we have a functioning part family generation prcess and seven well-characterized LacI promoter mutants and eight GFP mutants (two of which have been lovingly named "Orange-Mutated Green Fluorescent Protein," or "OMGfp" 1 and 2) which await further characterization.