We set out to develop a rugged process for the rapid production of mutant libraries of any BioBrick part using standard primers and a simple mutagenic PCR protocol. We chose to prototype this process by creating mutant libraries of the LacI, TetR and λ cI repressible promoters and to mutate GFP to visually assess our ability to create functional protein mutants.

As of October 2011, we have a functioning mutant library generation and screening process, and a selection of well-characterized promoter mutants, including seven LacI promoter mutants. We also have nine TetR promoter mutants, seven λ cI promoter mutants, and eight GFP mutants (two of which have been lovingly named "Orange-Mutated Green Fluorescent Protein," or "OMGfp" 1 and 2) which await further characterization.