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Team:Tec-Monterrey/projectnotebook may/day20
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PROJECT
20/May/2011 • DNA digestion with SspI and NdeI + Buffer 3 at 37 C , 1 hr. • Electrophoresis 60 V, 1.5 dr. • Pre-inocolum of virgin cells in 15-20 microliters of LB without antibiotic for calcium competent cells. • Purification of DNA of the agarose gel.
