Team:NYMU-Taipei/results/optomagnetic-design1
From 2011.igem.org
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Contents |
Constructs and Experimental Results
We now follow the steps we construct our design to examine and illustrate what we have done so far. The total parts we constructed and their detailed information will be performed and recorded in our partsregistry pages.
The Beginning
As soon as we have the AMB-1 bacteria colony, we do the AMB-1 colony PCR to get the Mms13's DNA sequences as our parts to do the following steps.
The correcterized gel electrophoresis result is shown below.(See Figure 2) The detail of part Mms13's information, please links to link:http://partsregistry.org/wiki/index.php?title=Part:BBa_K624005.
Construct Mms13
Then, after we have the fundamental material of Mms13, we did the next step of our construct.(See Figure 3)
The correcterized and checked results shown in Figure 4.
For parts mms13-rLuc, links to link:http://partsregistry.org/wiki/index.php?title=Part:BBa_K624007; YN-mms13-rLuc, links to link:http://partsregistry.org/wiki/index.php?title=Part:BBa_K624008; EYFP-mms13-rLuc fusion, please links to link:http://partsregistry.org/wiki/index.php?title=Part:BBa_K624006.
Construct CHAMP Design
As for the CHAMP part, we use ligation process to get the whole sequences of CHAMP peptides. However, we still use the recombinant PCR procedure to anchor either YC or YFP in our process. Several electrophoresis results for CHAMP constructs are shown below.
![](/wiki/images/1/17/Champ_NYMU.png)
![](/wiki/images/1/17/Champ_NYMU.png)
Whole Construct and Experimental Results
After we have both materials of Mms13's construction and CHAMP peptides' construction, we use ligation procedure to fulfill six constructs we design in our optomagnetic-design.
The correlated part of construct in Fig. 7 is pYMB essentials+[YN-13-R]+[YC-CHAMP], links to link:http://partsregistry.org/wiki/index.php?title=Part:BBa_K624018.
The related part of construct in Fig. 8 is pYMB essentials+[YN-13-R], links to http://partsregistry.org/wiki/index.php?title=Part:BBa_K624024.
![](/wiki/images/8/8d/C5_NYMU.png)