Team:NYMU-Taipei/results/optomagnetic-design1

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Contents

Six Constructs and Experimental Results

We now follow the steps we construct our design to examine and illustrate what we have done so far. The total parts we construct and their detailed information will be performed and recorded in our part registry.

Fig. 1:This is the total constructs of our optomagnetic design, the notation for each columns with different colors can be seen in the following paragraphs.

The Beginning

As soon as we have the AMB-1 bacteria colony, we do the AMB-1 colony PCR to get the Mms13's DNA sequences as our parts to do the following steps.

The correcterized gel electrophoresis result is shown below.(See Figure 2) The detail of Mms13's information, please links to link:http://partsregistry.org/wiki/index.php?title=Part:BBa_K624005.

Fig. 2:The correcterized gel electrophoresis result of Mms13.

Construct Mms13

Then, after we have the fundamental material of Mms13, we did the next step of our construct.(See Figure 3)

Fig. 3:We used several steps of recombinant PCR to assemble (1)mms13-rLuc； (2)YN-mms13-rLuc； (3)Y-mms13-rLuc, why we chose recombinant PCR is because of two reasons: (1) the less time it took and (2)we found better precision in recombinant PCR than in ligation process.

The correcterized and checked results shown in Figure 4.

Fig. 4:Checked YFP-Mms13-rluc part.

For parts mms13-rLuc, links to link:http://partsregistry.org/wiki/index.php?title=Part:BBa_K624007; YN-mms13-rLuc, links to http://partsregistry.org/wiki/index.php?title=Part:BBa_K624008; EYFP-mms13-rLuc fusion, please links to http://partsregistry.org/wiki/index.php?title=Part:BBa_K624006.

Construct CHAMP Design

As for the CHAMP part, we use ligation process to get the whole sequences of CHAMP peptides. However, we still use the recombinant PCR procedure to anchor either YC or YFP in our process. Several electrophoresis results for CHAMP constructs are shown below.

Fig. 5:Total CHAMP Ligation, and its part link to[http://partsregistry.org/wiki/index.php?title=Part:BBa_K624010]
Fig. 6:YFP with CHAMP Ligation sequences' N-terminus by recombinant PCR, and its associated parts-(1)CHAMP-YC, links to[http://partsregistry.org/wiki/index.php?title=Part:BBa_K624026], (2)CHAMP-EYFP, links to[http://partsregistry.org/wiki/index.php?title=Part:BBa_K624025].

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