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Team:Kyoto/Material
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Contents |
Materials
Strain(Fruits Fly)
| Name | Genotype and/or Relevant Features |
|---|---|
| D.melanogaster(fly) | Oregon-RS |
Strains(E.Coli)
| Name | Genotype and/or Relevant Features | Source or Reference |
|---|---|---|
| JM109 | endA, recA1, gyrA96,thi,hsdR17,(rκ-,mκ+),relA1, supE44,λ-,Δ(lac-proAB),[F',traD36,proAB,laclqZΔM15] | Promega[http://www.promega.com/products/cloning-and-dna-markers/cloning-tools-and-competent-cells/bacterial-strains-and-competent-cells/bacterial-strain-jm109_de3_/] |
| BW25113 | ∆(araD-araB)567, ∆lacZ4787(::rrnB-3), lambda-, rph-1, ∆(rhaD-rhaB)568, hsdR514 | E.coliwiki[http://ecoliwiki.net/colipedia/index.php/BW25113] |
We got BW25113 from NIG, National Institute of Genetics.
Strains(Streptomyces)
| Name | Genotype and/or Relevant Features | Source or Reference |
|---|---|---|
| Streptomyces avermitilis | MA-4680,ATCC 31267,BCRC 12382 | JCM Catalog[http://www.jcm.riken.jp/cgi-bin/jcm/jcm_keyword?AN=Streptomyces&BN=avermitilis&CN=&DN=] |
| Streptomyces albogriseolus | ATCC 23875, BCRC 12230 | JCM Catalog[http://www.jcm.riken.jp/cgi-bin/jcm/jcm_keyword?AN=Streptomyces&BN=albogriseolus&CN=&DN=] |
We got these strains from JCM, Japan Collection of Microorganisms.
Primers for PCR amplification
| Date | Name | Sequence |
|---|---|---|
| 07/21 | S Forward (Same as SRRz Forward) | gGAATTCGCGGCCGCTTCTAGAGgcctggatttgttctatcagtaatcga |
| 07/21 | S Reverse | CTGCAGCGGCCGCTACTAGTAttattgatttctaccatcttctactccggc |
| 07/21 | SRRz Reverse | CTGCAGCGGCCGCTACTAGTAtgagttgcccatcgatatgggc |
| 07/28 | ΔTMD1 Forward | cgcggcagatataatggcggt |
| 08/11 | ΔTMD1 Reverse | gtcatgtttttctggcatcttcatgtct |
| 08/11 | glnL Forward | tctagaggagactgctttatggcaac |
| 08/11 | glnL Reverse | actagtaggaactatcgtcatcgactac |
| 08/11 | glnG Forward | tctagaggtgacgtttatgcaacga |
| 08/11 | glnG Reverse | actagtacacacaagctgtgaatcactc |
| 08/19, 08/24 | Sam7 Forward | ggttcattcgtgaccttctcgacttc |
| 08/19, 08/24 | Sam7 Reverse | aggcgataatggcgcacatc |
| 08/20 | SRRz Forward Retry | gGAATTCGCGGCCGCTTCTAGAGgagcaaatccccttattgggggtaagacatg |
| 08/20 | SRRz Reverse Retry | CTGCAGCGGCCGCTACTAGTAgcaactctatctgcactgctcattaatatacttctgg |
| 08/23, 08/24 | Deletion of Upstream Forward | gagcaaatccccttattgggggtaagacatg |
| 08/23, 08/24 | Deletion of Upstream Reverse | ctctagaagcggccgcgaattcc |
| 08/23 | c-glnL Forward | gctctagaggagactgctttatggcaac |
| 08/23 | c-glnL Reverse | ggactagtaggaactatcgtcatcgactac |
| 08/23 | c-glnG Forward | gctctagaggtgacgtttatgcaacga |
| 08/23 | c-glnG Reverse | ggactagtacacacaagctgtgaatcactc |
| 08/26 | gln-promoter1 | cggaattcgcggccgcttctagagattgca |
| 08/26 | gln-promoter2r | gaatgttgcaccaatatagtgcttcaatggaaacattaagcaccatgttggtgcaatctctagaagcggc |
| 08/26 | gln-promoter3 | gaatgttgcaccaatatagtgcttcaatggaaacattaagcaccatgttggtgcaatctctagaagcggc |
| 08/26 | gln-promoter4r | ggactagtaaaagcgaaatctgtgccaacttttaaattgcccctaaaaggcgttatcatgcgcacc |
| 08/28 | cc-glnL Forward | ggaattcgcggccgcttctagaggagactgctttatggcaac |
| 08/28 | cc-glnG Forward | ggaattcgcggccgcttctagaggtgacgtttatgcaacga |
| 08/28 | rpoN Forward | ggaattcgcggccgcttctagaggttctgaacatgaagcaa |
| 08/28 | rpoN Reverse | ggactagtatccttatcggttgggtca |
| 08/31 | rpoN-Pstl-m2 | ggactagtagtagacgctgaccccgtag |
| 09/06 | rpoN-Pstl-m1 | gcaagatgccaaatggttgatcaagagtc |
| 09/12 | ChiA1 Forward | ggaattcgcggccgcttctagagattggtctggaccagtttgta |
| 09/12 | ChiA1 Reverse | ggactagtacactgccagttccacctacc |
| 09/12 | serine proteae Forward | ggaattcgcggccgcttctagagcctttgtggaggaacgaaac |
| 09/12 | serine proteae Reverse | ggactagtagtagacgctgaccccgtag |
| 09/13, 09/22 | Add J23105 Forward | CAGTCCTAGGTACTATGCTAGCtactagtagcggccgctgc |
| 09/13, 09/22 | Add J23105 Reverse | AGCTAGCCGTAAActctagtatataaacgcagaaaggcccacc |
| 10/10 | Deletion of GFP Forward (Primer1) | ccaggcatcaaataaaacgaaaggctc |
| 10/10 | Deletion of GFP-DT Forward (Primer2) | tactagtagcggccgctgc |
| 10/10 | Deletion of GFP, GFP-DT Reverse (Primer3) | ctctagtattattgatttctaccatcttctactcc |
| - | VF2 (<partinfo>G00100</partinfo>) | tgccacctgacgtctaagaa |
| - | VF (<partinfo>G00101</partinfo>) | attaccgcctttgagtgagc |
Primers for Real time PCR
| Date | Name | Sequence |
|---|---|---|
| 09/13 | sigma54-f | TTGATCCGGTCGGTGTG |
| 09/13 | sigma54-r | AGTGCGGAAGTCGTGATTG |
| 09/13 | GAPDH-f | GGTGCGAAGAAAGTGGTTATG |
| 09/13 | GAPDH-r | TGGTGCAGGAAGCGTTG |
| 09/13 | GUS-f | GGCAACAAGCCGAAAGAAC |
| 09/13 | GUS-r | GGCAATACTCCACATCACCAC |
| 09/13 | TBP-f | CAATTCATCTCGACCATCACAC |
| 09/13 | TBP-r | AAATCCACCACCTCTTTGACC |
| 09/13 | PGK-f | TCAACAAAGGCGAGAAGAAAG |
| 09/13 | actin-f | ACGGTGTGGTTTACTCCTCTTC |
| 09/13 | PGK-r | GAACCGATTTCGTGCTTGATAC |
| 09/23 | GUS-f | ACAGCCAAAAGCCAGACAG |
| 09/23 | GUS-r | TGACGACCAAAGCCAGTAAAG |
| 09/23 | TBP-f | ACGGCTCATGGGATGTATG |
| 09/23 | TBP-r | ATCCCCAGTTTGATGGTTTG |
| 09/23 | glnL1-f | GAGATGGCTCCGATGGATAAC |
| 09/23 | glnL1-r | ACTAAATCACGGGCAGCAAC |
| 09/23 | glnL2-f | TGCTGCCCGTGATTTAGTG |
| 09/23 | glnL2-r | ATTCGAGTAGTGATGGGTCAGG |
| 09/23 | glnL3-f | GATACGCTGTTTTACCCGATG |
| 09/23 | glnL3-r | GATAGGCAGGTAAACCGAGAAC |
| 09/23 | glnG1-f | GGCGGTACATTATTCCTCGAC |
| 09/23 | glnG1-r | TCCGCACATCCACTTTCAC |
| 09/23 | glnG2-f | TCAGCGCCTATCAACAAGG |
| 09/23 | glnG2-r | TGCTGCTGTTCCTGGTAATG |
| 09/23 | glnG3-f | CAGATTAAACAGCGCCATCC |
| 09/23 | glnG3-r | CAAACGCCCCTTGTTGATAG |
| 09/23 | sigma54promoter-rpoN-f | actaGAGGTTCTGaacatgaagc |
| 09/23 | sigma54promoter-rpoN-r | attactctccagcgcctgc |
| 09/23 | rpoN-glnLG-f | tactagaGGAGACTGCTTTatggc |
| 09/23 | rpoN-glnLG-r | cgccaggttgtcatcgattaac |
| 09/28 | bolA-f | CGTTTTCTGAATCGTCATCG |
| 09/28 | bolA-f | CGTTTTCTGAATCGTCATCG |
| 09/28 | bolA-r | ACAGGGAGGAGAGGCAAAG |
| 09/28 | csiE1-f | GCTCCACCATCTGTCCTTTC |
| 09/28 | csiE1-r | GGCAATATCATCATCCACACC |
| 09/28 | csi2-r | ATTGTCTGTCCCGCTGATG |
| 09/28 | dps-f | GGGACCACTCAAGTTATCAACAG |
| 09/28 | dps-r | GGTCAGCCAGTTCTTTCAGG |
