"
Team:Korea U Seoul/Notebook/Jul/passage
From 2011.igem.org
7/8 - Advisor's presentation
Advisor’s presentation
- Mechanisms
- Plans
- Things needed to be ordered
- -Vibrio harveyi; media check; KACC number check
- Things needed to be done by individual members
- -Analyze trends of supporting data
- -Detection methods by TLC, GC, HPLC, and understand the mechanism
- -Understand reasons for codon optimization
- Things needed to be done as a group
- -Group 1: Search for promoter and vector type
- -Group 2: Search for C14’s usage; cost, etc.
- -Group 3: Search for medium conditions
7/22 - Presentation on experiment
Group 1
- Presented about
- 1. Plasmid in which the gene will be incorporated
- 2. Promoter Tac = hybrid of E.coli's trp and lac operon
- - Strong promoter of gene expression
- 3. Induction can be done by the addition of IPTG(isopropyl-beta-D-1-thiolgalactopyranoside)
- - IPTG is a derivative of lactose and is used as an inducer to operate the promoter.
- 4. Repression can be done by the expression of lac1 protein, which is a type of repressor
- - lac1 protein is a DNA-binding protein involved in the metabolism of lactose.
- - Promoter mutation: intracellular concentration of lac1 repressor is increased, leading to stronger repression.
