| Name | Genotype and/or Relevant Features | Source or Reference
|
| JM109
| endA, recA1, gyrA96,thi,hsdR17,(rκ-,mκ+),relA1, supE44,λ-,Δ(lac-proAB),[F',traD36,proAB,laclqZΔM15]
| Promega[http://www.promega.com/products/cloning-and-dna-markers/cloning-tools-and-competent-cells/bacterial-strains-and-competent-cells/bacterial-strain-jm109_de3_/]
|
| BW25113
| ∆(araD-araB)567, ∆lacZ4787(::rrnB-3), lambda-, rph-1, ∆(rhaD-rhaB)568, hsdR514
| E.coliwiki[http://ecoliwiki.net/colipedia/index.php/BW25113]
|
| Date | Name | Sequence
|
| 07/21 | S Forward (Same as SRRz Forward) | gGAATTCGCGGCCGCTTCTAGAGgcctggatttgttctatcagtaatcga
|
| 07/21 | S Reverse | CTGCAGCGGCCGCTACTAGTAttattgatttctaccatcttctactccggc
|
| 07/21 | SRRz Reverse | CTGCAGCGGCCGCTACTAGTAtgagttgcccatcgatatgggc
|
| 07/28 | ΔTMD1 Forward | cgcggcagatataatggcggt
|
| 08/11 | ΔTMD1 Reverse | gtcatgtttttctggcatcttcatgtct
|
| 08/11 | glnL Forward | tctagaggagactgctttatggcaac
|
| 08/11 | glnL Reverse | actagtaggaactatcgtcatcgactac
|
| 08/11 | glnG Forward | tctagaggtgacgtttatgcaacga
|
| 08/11 | glnG Reverse | actagtacacacaagctgtgaatcactc
|
| 08/19, 08/24 | Sam7 Forward | ggttcattcgtgaccttctcgacttc
|
| 08/19, 08/24 | Sam7 Reverse | aggcgataatggcgcacatc
|
| 08/20 | SRRz Forward Retry | gGAATTCGCGGCCGCTTCTAGAGgagcaaatccccttattgggggtaagacatg
|
| 08/20 | SRRz Reverse Retry | CTGCAGCGGCCGCTACTAGTAgcaactctatctgcactgctcattaatatacttctgg
|
| 08/23, 08/24 | Deletion of Upstream Forward | gagcaaatccccttattgggggtaagacatg
|
| 08/23, 08/24 | Deletion of Upstream Reverse | ctctagaagcggccgcgaattcc
|
| 08/23 | c-glnL Forward | gctctagaggagactgctttatggcaac
|
| 08/23 | c-glnL Reverse | ggactagtaggaactatcgtcatcgactac
|
| 08/23 | c-glnG Forward | gctctagaggtgacgtttatgcaacga
|
| 08/23 | c-glnG Reverse | ggactagtacacacaagctgtgaatcactc
|
| 08/26 | gln-promoter1 | cggaattcgcggccgcttctagagattgca
|
| 08/26 | gln-promoter2r | gaatgttgcaccaatatagtgcttcaatggaaacattaagcaccatgttggtgcaatctctagaagcggc
|
| 08/26 | gln-promoter3 | gaatgttgcaccaatatagtgcttcaatggaaacattaagcaccatgttggtgcaatctctagaagcggc
|
| 08/26 | gln-promoter4r | ggactagtaaaagcgaaatctgtgccaacttttaaattgcccctaaaaggcgttatcatgcgcacc
|
| 08/28 | cc-glnL Forward | ggaattcgcggccgcttctagaggagactgctttatggcaac
|
| 08/28 | cc-glnG Forward | ggaattcgcggccgcttctagaggtgacgtttatgcaacga
|
| 09/12 | ChiA1 Forward | ggaattcgcggccgcttctagagattggtctggaccagtttgta
|
| 09/12 | ChiA1 Reverse | ggactagtacactgccagttccacctacc
|
| 09/12 | serine proteae Forward | ggaattcgcggccgcttctagagcctttgtggaggaacgaaac
|
| 09/12 | serine proteae Reverse | ggactagtagtagacgctgaccccgtag
|
| 09/13, 09/22 | Add J23105 Forward | CAGTCCTAGGTACTATGCTAGCtactagtagcggccgctgc
|
| 09/13, 09/22 | Add J23105 Reverse | AGCTAGCCGTAAActctagtatataaacgcagaaaggcccacc
|
| 10/10 | Deletion of GFP Forward (Primer1) | ccaggcatcaaataaaacgaaaggctc
|
| 10/10 | Deletion of GFP-DT Forward (Primer2) | tactagtagcggccgctgc
|
| 10/10 | Deletion of GFP, GFP-DT Reverse (Primer3) | ctctagtattattgatttctaccatcttctactcc
|
| - | VF2 (<partinfo>G00100</partinfo>) | tgccacctgacgtctaagaa
|
| - | VF (<partinfo>G00101</partinfo>) | attaccgcctttgagtgagc
|
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