Team:Tokyo Metropolitan/Notebook/S06

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Speeding Up

Colony PCR of HNS(wt)+I20260. Confirmed amplifing DNA but length of fragment was incorrect.

Electrophoresis of digested HNS(wt)(XbaI+SpeI),I20260(BlnⅠ).Concentration of both HNS(wt)(XbaI+SpeI) and I20260(BlnⅠ) was low.

Preculture(pSB1C3)

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