Team:NYMU-Taipei/chassis

Cultivating Magnetospirillum magneticum AMB-1 Actually, it wasn't difficult...

Like E-coli, AMB-1 also grows in liquid medium, and this medium called Magnetic Spirillum Growth Medium (MSGM). In the medium, we added nutrient, iron source (supplying AMB-1 in magnetite formation within magnetosomes), vitamin, and mineral solution.

MSGM's ingredients

(In grams per liter)

●Succinic acid             0.74 g

●KH2PO4                    0.68 g

●Sodium nitrate            0.12 g

●Sodium thioglycolate      0.1 g

●Wolfe’s vitamin solution  10 ml(stock)

●Wolfe’s mineral solution  5  ml(stock)

●Ferric quinate solution   2  ml(0.27 g of FeCl3 and 0.19 g of quinic acid in 100 ml water)

We also refered a paper which added Polypeptone , Yeast extract into MSGM and added L-cysteine instead of sodium thioglycolate . Which called En-rich MSGM.In <<Effects of growth medium composition, iron sources and atmospheric oxygen concentrations on production of luciferase-bacterial magnetic particle complex by a recombinant Magnetospirillum magneticum AMB-1,2001>> said that MSGM enriched with L-cysteine, yeast extract and polypeptone could enhance BMP productivity. Addition of yeast extract had no effect on BMP production and polypeptone only improved the final cell density and therefore slightly improved BMP production, whereas L-cysteine induced cell growth!

En-rich MSGM's ingredients

(In grams per liter)

●Succinic acid             0.74 g

●KH2PO4                    0.68 g

●Sodium nitrate            0.12 g

●Wolfe’s vitamin solution  10 ml(stock)

●Wolfe’s mineral solution  5  ml(stock)

●Ferric quinate solution   2  ml(0.27 g of FeCl3 and 0.19 g of quinic acid in 100 ml water)

●L-cysteine                0.005%

●Yeast extract             0.01%'

●Polypeptone               0.02%

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