Team:UQ-Australia/Parts

From 2011.igem.org

Revision as of 08:41, 4 October 2011 by Akashboda (Talk | contribs)




The human circadian rhythm drives many important processes in the body in accordance with the sleep/wake cycle. A characteristic of this biological clock is the periodic oscillation of gene expression. Current parts in the Registry designed to regulate periodic oscillations of gene expression have shown limited success.

Here we demonstrate a biological clock being standardised as a set of BioBrick parts.

IGEM basic Logo stylized.png

Our network is controlled by an engineered promoter, Plac/ara, which features both an activator and a repressor domain. This controls the production of downstream genes to activate other inducible promoters, pBAD and GlnAp2, eventually leading to the production of a repressor protein, lacI, which inhibits Plac/ara, resulting in oscillatory expression. This project shows the feasibility of standardising the biological clock in E. coli and grounds further development for applications in regulated drug/hormone delivery and ion channel control.

UQ-Australia logo 2011.png



Parts

Students

Aakash.jpg Plac/ara

Third year Bachelor of Biomedical Sciences majoring in Neurobiology and Developmental Science

Akash.jpg glnG

Third year Bachelor of Biotechnology majoring in Bioinformatics

125x125px araC

Third year Bachelor of Science majoring in Physics

125x125px pBAD

Third year Bachelor of Biotechnology majoring in Molecular Biotechnology

125x125px LacI

Third year Bachelor of Biomedical Sciences majoring in Neuroscience

Josh.jpg GlnAp2

Third year Bachelor of Biomedical Sciences majoring in Human Genetics and Biomedical Science


New for iGEM 2010 is the groupparts tag. This tag will generate a table with all of the parts that your team adds to your team sandbox. Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.

<groupparts>iGEM010 UQ-Australia</groupparts>